A DNA adenine demethylase impairs PRC2-mediated repression of genes marked by a specific chromatin signature.

ABSTRACT:

Background

The Fe (II)- and α-ketoglutarate-dependent AlkB family dioxygenases are implicated in nucleotide demethylation. AlkB homolog1 (ALKBH1) is shown to demethylate DNA adenine methylation (6mA) preferentially from single-stranded or unpaired DNA, while its demethylase activity and function in the chromatin context are unclear.

Results

Here, we find that loss-of-function of the rice ALKBH1 gene leads to increased 6mA in the R-loop regions of the genome but has a limited effect on the overall 6mA level. However, in the context of mixed tissues, rather than on individual loci, the ALKBH1 mutation or overexpression mainly affects the expression of genes with a specific combination of chromatin modifications in the body region marked with H3K4me3 and H3K27me3 but depleted o

SUBMITTER: Jia Q 

PROVIDER: S-EPMC10469495 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature