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Imaging and quantitative analysis of integrin-dependent cell-matrix adhesions.

ABSTRACT: Integrin-dependent cell-extracellular matrix adhesion is essential for wound healing, embryonic development, immunity, and tissue organization. Here, we present a protocol for the imaging and quantitative analysis of integrin-dependent cell-matrix adhesions. We describe steps for cell culture; virus preparation; lentiviral transduction; imaging with widefield, confocal, and total internal reflection fluorescence microscopy; and using a script for their quantitative analysis. We then detail procedures for analyzing adhesion dynamics by live-cell imaging and fluorescence recovery after photobleaching (FRAP). For complete details on the use and execution of this protocol, please refer to Margadant et al. (2012),¹ van der Bijl et al. (2020),² Amado-Azevedo et al. (2021).³.

SUBMITTER: van Stalborch AD

PROVIDER: S-EPMC10469561 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Imaging and quantitative analysis of integrin-dependent cell-matrix adhesions.

van Stalborch Anne-Marieke D AD Clark Andrew G AG Sonnenberg Arnoud A Margadant Coert C

STAR protocols 20230823 3

Integrin-dependent cell-extracellular matrix adhesion is essential for wound healing, embryonic development, immunity, and tissue organization. Here, we present a protocol for the imaging and quantitative analysis of integrin-dependent cell-matrix adhesions. We describe steps for cell culture; virus preparation; lentiviral transduction; imaging with widefield, confocal, and total internal reflection fluorescence microscopy; and using a script for their quantitative analysis. We then detail proce ...[more]

PMID: 37616164

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