Maximizing the potential of high-throughput next-generation sequencing through precise normalization based on read count distribution.

ABSTRACT: Next-generation sequencing technologies have enabled many advances across diverse areas of biology, with many benefiting from increased sample size. Although the cost of running next-generation sequencing instruments has dropped substantially over time, the cost of sample preparation methods has lagged behind. To counter this, researchers have adapted library miniaturization protocols and large sample pools to maximize the number of samples that can be prepared by a certain amount of reagents and sequenced in a single run. However, due to high variability of sample quality, over and underrepresentation of samples in a sequencing run has become a major issue in high-throughput sequencing. This leads to misinterpretation of results due to increased noise, and additional time and cost rerunni

SUBMITTER: Brennan C 

PROVIDER: S-EPMC10469589 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature