Quantitative differences in synthetic gut microbial inoculums do not affect the final stabilized in vitro community compositions.

ABSTRACT: In vitro studies of synthetic gut microbial communities (SGMCs) can provide valuable insights into the ecological structure and function of gut microbiota. However, the importance of the quantitative composition of an SGMC inoculum and its effect on the eventual stable in vitro microbial community has not been studied. To address this, we constructed two 114-member SGMCs differing only in their quantitative composition-one reflecting the average human fecal microbiome and another mixed in equal proportions based on cell counts. We inoculated each in an automated anaerobic multi-stage in vitro gut fermentor simulating two different colonic conditions, mimicking proximal and distal colons. We replicated this setup with two different nutrient media, periodically sampled t

SUBMITTER: Gnanasekaran T 

PROVIDER: S-EPMC10469597 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature