Glucose metabolism and function of CD4⁺ Tregs are regulated by the TLR8/mTOR signal in an environment of SKOV3 cell growth.

ABSTRACT:

Purpose

To investigate the role of mammalian target of rapamycin (mTOR) signal in Toll-like receptor (TLR) 8-mediated regulation of glucose metabolism and its effect on reversing immunosuppression in CD4⁺ regulatory T-cells (Tregs) in ovarian cancer (OC).

Methods

Fluorescence-activated cell sorting was used to detect the expression levels of mTOR⁺ and 4E-BP1⁺ cells in CD4⁺ Tregs. The prognosis and immune infiltration analysis of mTOR mRNA in OC were performed using the TIMER and Kaplan-Meier plotter database. Furthermore, real-time polymerase chain reaction (RT-PCR) and western blot (WB) were used to detect expression levels of glucose metabolism-related genes and proteins in CD4⁺ Tregs. Glucose uptake and glycolysis lev

SUBMITTER: Wu M 

PROVIDER: S-EPMC10469653 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature