Prostate cancer cells synergistically defend against CD8⁺ T cells by secreting exosomal PD-L1.

ABSTRACT:

Background

Metastatic castration-resistant prostate cancer (mCRPC) remains fatal and incurable, despite a variety of treatments that can delay disease progression and prolong life. Immune checkpoint therapy is a promising treatment. However, emerging evidence suggests that exosomal programmed necrosis ligand 1 (PD-L1) directly binds to PD-1 on the surface of T cells in the drain lineage lymph nodes or neutralizes administered PD-L1 antibodies, resulting in poor response to anti-PD-L1 therapy in mCRPC.

Materials and methods

Western blotting and immunofluorescence were performed to compare PD-L1 levels in exosomes derived from different prostate cancer cells. PC3 cells were subcutaneously injected into nude mice, and then ELISA assay was used to detect human specific PD-L1 in

SUBMITTER: Li D 

PROVIDER: S-EPMC10469662 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature