Inhibitory to non-inhibitory evolution of the ζ subunit of the F₁F_O-ATPase of Paracoccus denitrificans and α-proteobacteria as related to mitochondrial endosymbiosis.

ABSTRACT: Introduction: The ζ subunit is a potent inhibitor of the F₁F_O-ATPase of Paracoccus denitrificans (PdF₁F_O-ATPase) and related α-proteobacteria different from the other two canonical inhibitors of bacterial (ε) and mitochondrial (IF₁) F₁F_O-ATPases. ζ mimics mitochondrial IF₁ in its inhibitory N-terminus, blocking the PdF₁F_O-ATPase activity as a unidirectional pawl-ratchet and allowing the PdF₁F_O-ATP synthase turnover. ζ is essential for the respiratory growth of P. denitrificans, as we showed by a Δζ knockout. Given the vital role of ζ in the physiology of P. denitrificans, here, we assessed t

SUBMITTER: Mendoza-Hoffmann F 

PROVIDER: S-EPMC10469736 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature