Cuproptosis-related miRNAs signature and immune infiltration characteristics in colorectal cancer.

ABSTRACT:

Background

A novel form of cell death termed cuproptosis was proposed recently. miRNAs play important roles in colorectal cancer (CRC). However, their relationships have not been reported.

Methods

miRNAs that negatively regulate 16 cuproptosis regulators were predicted using Targetscan database. The univariate Cox, LASSO, and multivariate Cox regression analyses were performed to select cuproptosis-related miRNAs. GSEA and ssGSEA analysis was carried out for functional enrichment analysis. The immune cell proportion score (IPS) and the efficiencies of multiple chemotherapy drugs were compared between different risk groups. The CCK8, cell colony, edu, and flow cytometry assays were performed to validate the roles of miRNA. Luciferase reporter assay confirmed the regulatory me

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC10469834 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature