Elucidating the CodY regulon in Staphylococcus aureus USA300 substrains TCH1516 and LAC.

ABSTRACT: CodY is a conserved broad-acting transcription factor that regulates the expression of genes related to amino acid metabolism and virulence in Gram-positive bacteria. Here, we performed the first in vivo determination of CodY target genes using a novel CodY monoclonal antibody in methicillin-resistant Staphylococcus aureus (MRSA) USA300. Our results showed (i) the same 135 CodY promoter binding sites regulating the 165 target genes identified in two closely related virulent S. aureus USA300 TCH1516 and LAC strains; (ii) the differential binding intensity for the same target genes under the same conditions was due to sequence differences in the same CodY-binding site in the two strains; (iii) a CodY regulon comprising 72 target genes that are differentially regulated re

SUBMITTER: Gao Y 

PROVIDER: S-EPMC10470025 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature