DNA and histones impair the mechanical stability and lytic susceptibility of fibrin formed by staphylocoagulase.

ABSTRACT:

Background

Staphylocoagulase (SCG) is a virulence factor of Staphylococcus aureus, one of the most lethal pathogens of our times. The complex of SCG with prothrombin (SCG/ProT) can clot fibrinogen, and SCG/ProT-induced fibrin and plasma clots have been described to show decreased mechanical and lytic resistance, which may contribute to septic emboli from infected cardiac vegetations. At infection sites, neutrophils can release DNA and histones, as parts of neutrophil extracellular traps (NETs), which in turn favor thrombosis, inhibit fibrinolysis and strengthen clot structure.

Objectives

To characterize the combined effects of major NET-components (DNA, histone H1 and H3) on SCG/ProT-induced clot structure, mechanical and lytic stability.

Methods

Recombinant SC

SUBMITTER: Komorowicz E 

PROVIDER: S-EPMC10470048 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature