Real-time detection of virus antibody interaction by label-free common-path interferometry.

ABSTRACT: Viruses have a profound influence on all forms of life, motivating the development of rapid and minimally invasive methods for virus detection. In this study, we present a novel methodology that enables quantitative measurement of the interaction between individual biotic nanoparticles and antibodies in solution. Our approach employs a label-free, full-field common-path interferometric technique to detect and track biotic nanoparticles and their interactions with antibodies. It is based on the interferometric detection of light scattered by viruses in aqueous samples for the detection of individual viruses. We employ single-particle tracking analysis to characterize the size and properties of the detected nanoparticles, and to monitor the changes in their diffusive mobility resulting from

SUBMITTER: Alhaddad S 

PROVIDER: S-EPMC10470184 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature