Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody.

ABSTRACT: As a highly pathogenic large DNA virus, African swine fever virus (ASFV) has huge particles and numerous encoded proteins. At present, few of the existing studies on ASFV proteins have investigated the function of p17. Specific antibodies against p17 to promote the development of prevention techniques against African swine fever (ASF) are urgently needed. Herein, we successfully expressed ASFV p17 in CHO cells using a suspension culture system and generated a monoclonal antibody (mAb) against p17. The mAb recognized a novel linear epitope (⁸LLSHNLSTREGIK²⁰) and exhibited specific reactivity, which was conducive to the identification of ectopically expressed p17, the recombinant porcine reproductive and respiratory syndrome virus expressing p17, and the ASFV-SY18. The

SUBMITTER: Li L 

PROVIDER: S-EPMC10470389 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature