Sonic Hedgehog and WNT Signaling Regulate a Positive Feedback Loop Between Intestinal Epithelial and Stromal Cells to Promote Epithelial Regeneration.

ABSTRACT:

Background and aims

Active intestinal stem cells are prone to injury by ionizing radiation. We previously showed that upon radiation-induced injury, normally quiescent reserve intestinal stem cells (rISCs) (marked by BMI1) are activated by Musashi-1 (MSI1) and exit from the quiescent state to regenerate the intestinal epithelium. This study aims to further establish the mechanism that regulates activation of Bmi1-Cre^ER;Rosa26^eYFP (Bmi1-Cre^ER) rISCs following γ radiation-induced injury.

Methods

Bmi1-Cre^ER mice were treated with tamoxifen to initiate lineage tracing of BMI1 (eYFP⁺) cells and exposed to 12 Gy of total body γ irradiation or sham. Intestinal tissues were collected and analyzed by immunofluorescence, Western b

SUBMITTER: Orzechowska-Licari EJ 

PROVIDER: S-EPMC10470419 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature