Transcriptional activation of ompA in Neisseria gonorrhoeae mediated by the XRE family member protein NceR.

ABSTRACT: Increasing antibiotic resistance of Neisseria gonorrhoeae, the causative agent of gonorrhea, is a growing global concern that has renewed vaccine development efforts. The gonococcal OmpA protein was previously identified as a vaccine candidate due to its surface exposure, conservation, stable expression, and involvement in host-cell interactions. We previously demonstrated that the transcription of ompA can be activated by the MisR/MisS two-component system. Interestingly, earlier work suggested that the availability of free iron also influences ompA expression, which we confirmed in this study. In the present study, we found that iron regulation of ompA was independent of MisR and searched for additional regulators. A DNA pull-down assay with the ompA pr

SUBMITTER: Holley CL 

PROVIDER: S-EPMC10470515 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature