A DNA phosphorothioation-based Dnd defense system provides resistance against various phages and is compatible with the Ssp defense system.

ABSTRACT: DndABCDE-catalyzed DNA phosphorothioation (PT), in which the nonbridging oxygen is swapped with a sulfur atom, was first identified in the bacterial genome. Usually, this modification gene cluster is paired with a restriction module consisting of DndF, DndG, and DndH. Although the mechanisms for the antiphage activity conferred by this Dnd-related restriction and modification (R-M) system have been well characterized, several features remain unclear, including the antiphage spectrum and potential interference with DNA methylation. Recently, a novel PT-related R-M system, composed of the modification module SspABCD paired with a single restriction enzyme, SspE, was revealed to be widespread in the bacterial kingdom, which aroused our interest in the interaction between Dnd- and Ssp-based R-

SUBMITTER: Jiang S 

PROVIDER: S-EPMC10470545 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature