Differential Expression of the β3 Subunit of Voltage-Gated Ca²⁺ Channel in Mesial Temporal Lobe Epilepsy.

ABSTRACT: The purpose of this study was to identify and validate new putative lead drug targets in drug-resistant mesial temporal lobe epilepsy (mTLE) starting from differentially expressed genes (DEGs) previously identified in mTLE in humans by transcriptome analysis. We identified consensus DEGs among two independent mTLE transcriptome datasets and assigned them status as "lead target" if they (1) were involved in neuronal excitability, (2) were new in mTLE, and (3) were druggable. For this, we created a consensus DEG network in STRING and annotated it with information from the DISEASES database and the Target Central Resource Database (TCRD). Next, we attempted to validate lead targets using qPCR, immunohistochemistry, and Western blot on hippocampal and temporal lobe neocortical tissue from mTLE

SUBMITTER: Kjær C 

PROVIDER: S-EPMC10471638 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature