Developmental reprogramming of myometrial stem cells by endocrine disruptor linking to risk of uterine fibroids.

ABSTRACT:

Background

The stage, when tissues and organs are growing, is very vulnerable to environmental influences, but it's not clear how exposure during this time causes changes to the epigenome and increases the risk of hormone-related illnesses like uterine fibroids (UFs).

Methods

Developmental reprogramming of myometrial stem cells (MMSCs), the putative origin from which UFs originate, was investigated in vitro and in the Eker rat model by RNA-seq, ChIP-seq, RRBS, gain/loss of function analysis, and luciferase activity assays.

Results

When exposed to the endocrine-disrupting chemical (EDC) diethylstilbestrol during Eker rat development, MMSCs undergo a reprogramming of their estrogen-responsive transcriptome. The reprogrammed genes in MMSCs are known as estrogen-responsiv

SUBMITTER: Yang Q 

PROVIDER: S-EPMC10471700 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature