Targeted inhibition of eIF5A^hpu suppresses tumor growth and polarization of M2-like tumor-associated macrophages in oral cancer.

ABSTRACT: Eukaryotic initiation factor 5A2 (eIF5A2) is overexpressed in many types of cancer, and spermidine-mediated eIF5A hypusination (eIF5A^hpu) appears to be essential to most of eIF5A's biological functions, including its important role in regulating cancer cell proliferation, epithelial-mesenchymal transition (EMT), and cancer stem cell (CSC) properties as well as immune cell functions. Here we investigated the role of eIF5A^hpu in the growth of oral squamous cell carcinoma cells (OSCCs) and OSCC-induced polarization of M2-like tumor-associated macrophages (TAMs). TCGA dataset analysis revealed an overall upregulation in the mRNA expression of eIF5A2 and several key enzymes involved in polyamine (PA) metabolism in HNSCC, which was confirmed by Western blot and IHC studies.

SUBMITTER: Zeng J 

PROVIDER: S-EPMC10471704 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature