Location of CD39⁺ T cell subpopulations within tumors predict differential outcomes in non-small cell lung cancer.

ABSTRACT:

Purpose

An improved mechanistic understanding of immunosuppressive pathways in non-small cell lung cancer (NSCLC) is important to develop novel diagnostic and therapeutic approaches. Here, we investigate the prognostic significance of the ectonucleotidases CD39 and CD73 in NSCLC.

Experimental design

The expression and localization of CD39, CD73 and CD103 was digitally quantified in a cohort of 162 early treatment naïve NSCLC patients using multiplex-immunofluorescence and related to patient outcome. Expression among different cell-populations was assessed via flow cytometry. Targeted RNA-Seq was performed on CD4⁺ and CD8⁺ T cells from digested NSCLC tumor tissue and single-cell RNA-Seq data was analyzed to investigate the functional significance of CD39

SUBMITTER: Koppensteiner L 

PROVIDER: S-EPMC10471883 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature