Decellularised spinal cord matrix manipulates glial niche into repairing phase via serglycin-mediated signalling pathway.

ABSTRACT: Astrocytes are the most abundant and widespread glial cells in the central nervous system. The heterogeneity of astrocytes plays an essential role in spinal cord injury (SCI) repair. Decellularised spinal cord matrix (DSCM) is advantageous for repairing SCI, but little is known regarding the exact mechanisms and niche alterations. Here, we investigated the DSCM regulatory mechanism of glial niche in the neuro-glial-vascular unit using single-cell RNA sequencing. Our single cell sequencing, molecular and biochemical experiments validated that DSCM facilitated the differentiation of neural progenitor cells through increasing the number of immature astrocytes. Upregulation of mesenchyme-related genes, which maintained astrocyte immaturity, causing insensitivity to inflammatory stimuli. Subseq

SUBMITTER: Zhang S 

PROVIDER: S-EPMC10472524 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature