M⁶A transcriptome-wide map of circRNAs identified in the testis of normal and AZ-treated Xenopus laevis.

ABSTRACT:

Background

Evidence showed that N⁶-methyladenosine (m⁶A) is strongly associated with male germline development. However, the role of m⁶A methylation on circRNAs in amphibians remains unknown. In this study, we conducted m⁶A sequencing analysis to explore the m⁶A transcriptome-wide profile of circRNAs in testis tissues of Xenopus laevis (X. laevis) with and without treatment with 100 µg/L atrazine (AZ).

Results

The analysis showed that m⁶A modification of circRNAs enriched in sense overlapping in testes of X. laevis. We identified the differential m⁶A modification sites within circRNAs in testes of AZ-exposed X. laevis and compared that with animals from control group. The results showed that a total

SUBMITTER: Zhang X 

PROVIDER: S-EPMC10472591 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature