Effects of host species on microbiota composition in Phlebotomus and Lutzomyia sand flies.

ABSTRACT:

Background

Blood-sucking phlebotomine sand flies are vectors of the protozoan parasites Leishmania spp. Although the intestinal microbiota is involved in a wide range of biological and physiological processes and has the potential to alter vector competence, little is known about the factors that modify the gut microbiota composition of sand flies. As a key step toward addressing this issue, we investigated the impact of host species on the gut bacterial composition in Phlebotomus and Lutzomyia sand flies reared under the same conditions.

Methods

Bacterial 16S rRNA gene amplification and Illumina MiSeq sequencing were used to characterize the overall bacterial composition of three laboratory-reared sandflies: Phlebotomus papatasi, Ph. duboscqi, and Lutzomyia longipalpis.

SUBMITTER: Tabbabi A 

PROVIDER: S-EPMC10472604 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature