Urine proteomics by mass spectrometry identifies proteins involved in key pathogenic pathways in patients with juvenile dermatomyositis.

ABSTRACT:

Objectives

To identify and validate biomarkers in JDM patients using a multiplexing tandem mass tag urine proteome profiling approach.

Methods

First morning void urine samples were collected from JDM patients (n = 20) and healthy control subjects (n = 21) and processed for analysis using a standardized liquid chromatography-tandem mass spectrometry approach. Biomarkers with significantly altered levels were correlated with clinical measures of myositis disease activity and damage. A subset of candidate biomarkers was validated using commercially available ELISA kits.

Results

In total, 2348 proteins were detected in the samples, with 275 proteins quantified across all samples. Among the differentially altered proteins, cathepsin D and galectin-3 binding protein were si

SUBMITTER: Morales M 

PROVIDER: S-EPMC10473190 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature