Recombinant anti-human epidermal growth factor receptor type 2 single-chain variable fragment-alpha-luffin fusion protein as a putative immunotoxin against human epidermal growth factor receptor type 2-positive breast cancer cells: an experimental research.

ABSTRACT:

Background

Breast cancer is one of the most frequent causes of cancer death in women. The application of immunotoxins to target overexpressed biomarkers on the surface of cancer cells and delivery of the toxin molecules into these cells has attracted too much attention during the last decade.

Objectives

This study was conducted to investigate the possible in-vitro cytotoxic and apoptotic activity of previously designed recombinant immunotoxin compromising anti-HER2 single-chain variable fragment (scFv) and alpha-luffin protein in human epidermal growth factor receptor type 2 (HER2)-positive and HER2-negative breast cancer cell lines.

Materials and methods

The previously designed recombinant immunotoxin and alpha-luffin protein were expressed in E. coli host cel

SUBMITTER: Damough S 

PROVIDER: S-EPMC10473382 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature