In-silico and in-vitro morphometric analysis of intestinal organoids.

ABSTRACT: Organoids offer a powerful model to study cellular self-organisation, the growth of specific tissue morphologies in-vitro, and to assess potential medical therapies. However, the intrinsic mechanisms of these systems are not entirely understood yet, which can result in variability of organoids due to differences in culture conditions and basement membrane extracts used. Improving the standardisation of organoid cultures is essential for their implementation in clinical protocols. Developing tools to assess and predict the behaviour of these systems may produce a more robust and standardised biological model to perform accurate clinical studies. Here, we developed an algorithm to automate crypt-like structure counting on intestinal organoids in both in-vitro and in-silico images. In additio

SUBMITTER: Montes-Olivas S 

PROVIDER: S-EPMC10473498 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature