A Biotin Targeting Chimera (BioTAC) System to Map Small Molecule Interactomes <i>in situ</i>.

ABSTRACT: Unbiased chemical biology strategies for direct readout of protein interactome remodelling by small molecules provide advantages over target-focused approaches, including the ability to detect previously unknown targets, and the inclusion of chemical off-compete controls leading to high-confidence identifications. We describe the BioTAC system, a small-molecule guided proximity labelling platform, to rapidly identify both direct and complexed small molecule binding proteins. The BioTAC system overcomes a limitation of current approaches, and supports identification of both inhibitor bound and molecular glue bound complexes.

SUBMITTER: Tao AJ 

PROVIDER: S-EPMC10473607 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature