Efficient and multiplexed tracking of single cells using whole-body PET/CT.

ABSTRACT: In vivo molecular imaging tools are crucially important for elucidating how cells move through complex biological systems, however, achieving single-cell sensitivity over the entire body remains challenging. Here, we report a highly sensitive and multiplexed approach for tracking upwards of 20 single cells simultaneously in the same subject using positron emission tomography (PET). The method relies on a new tracking algorithm (PEPT-EM) to push the cellular detection threshold to below 4 Bq/cell, and a streamlined workflow to reliably label single cells with over 50 Bq/cell of ¹⁸F-fluorodeoxyglucose (FDG). To demonstrate the potential of method, we tracked the fate of over 70 melanoma cells after intracardiac injection and found they primarily arrested in the small capill

SUBMITTER: Nguyen HTM 

PROVIDER: S-EPMC10473747 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature