Female germline expression of OVO transcription factor bridges Drosophila generations.

ABSTRACT: OVO is required for karyotypically female germ cell viability but has no known function in the male germline in Drosophila. ovo is autoregulated by two antagonistic isoforms, OVO-A and OVO-B. All ovo^- alleles were created as partial revertants of the antimorphic ovo^D1 allele. Creation of new targeted alleles in an ovo⁺ background indicated that disrupting the germline-specific exon extension of ovo-B leads to an arrested egg chamber phenotype, rather than germ cell death. RNA-seq analysis, including >1K full length cDNAs, indicates that ovo utilizes a number of unannotated splice variations in the extended exon and a minor population of ovo-B transcripts utilizes an alternative splice. This indicates t

SUBMITTER: Benner L 

PROVIDER: S-EPMC10473757 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature