Lin28a maintains a subset of adult muscle stem cells in an embryonic-like state.

ABSTRACT: During homeostasis and after injury, adult muscle stem cells (MuSCs) activate to mediate muscle regeneration. However, much remains unclear regarding the heterogeneous capacity of MuSCs for self-renewal and regeneration. Here, we show that Lin28a is expressed in embryonic limb bud muscle progenitors, and that a rare reserve subset of Lin28a⁺Pax7^- skeletal MuSCs can respond to injury at adult stage by replenishing the Pax7⁺ MuSC pool to drive muscle regeneration. Compared with adult Pax7⁺ MuSCs, Lin28a⁺ MuSCs displayed enhanced myogenic potency in vitro and in vivo upon transplantation. The epigenome of adult Lin28a⁺ MuSCs showed resemblance to embryonic muscle progenitors. In addition, RNA-sequencing revealed that Lin28a

SUBMITTER: Wang P 

PROVIDER: S-EPMC10474071 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature