Challenges in the determination of total vitamin B12 by cyanidation conversion: insights from stable isotope dilution assays.

ABSTRACT: Previous methods for vitamin B12 (B12) analysis have extensively used cyanidation conversion with the intention of converting all cobalamins to cyanocobalamin (CNCbl) for total B12 determination. This approach has been favored for its advantages in reducing the number of analytes, increasing analyte concentration, and improving analyte stability. However, the present study revealed underlying limitations associated with this approach. First, a stable isotope dilution assay (SIDA) determining total B12 as CNCbl after cyanidation conversion (conversion SIDA method) was developed. Method validation demonstrated good sensitivity, recovery, accuracy, and reproducibility for the target analyte CNCbl. However, subsequent application of the conversion method to real meat samples showed incomplete

SUBMITTER: Wang M 

PROVIDER: S-EPMC10474169 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature