Application of a modified tetra-primer ARMS-PCR assay for rapid Panax species identity authentication in ginseng products.

ABSTRACT: Panax ginseng products can be adulterated with materials from other Panax species. The purpose of this study is to provide a rapid P. ginseng authentication method for simultaneous identification of P. ginseng and detection of adulteration in ginseng products at different processing stages. First, a tetra-primer ARMS-PCR assay was designed based on a single-nucleotide polymorphism (SNP) within the trnL-trnF region and was tested at 28 PCR cycles with DNA extracted from Botanical Reference Materials (BRMs). Next, 5' end random nucleotide and 3' terminus phosphorothioates linkage modifications were incorporated into the inner primers to improve sensitivity and specificity at 40 PCR cycles. Finally, the modified assay was validated using characterized market ginseng materials and the detectio

SUBMITTER: Yang Z 

PROVIDER: S-EPMC10474259 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature