Sex differences in distribution and identity of aromatase gene expressing cells in the young adult rat brain.

ABSTRACT:

Background

Aromatase catalyzes the synthesis of estrogens from androgens. Knowledge on its regional expression in the brain is of relevance to the behavioral implications of these hormones that might be linked to sex differences in mental health. The present study investigated the distribution of cells expressing the aromatase coding gene (Cyp19a1) in limbic regions of young adult rats of both sexes, and characterized the cell types expressing this gene.

Methods

Cyp19a1 mRNA was mapped using fluorescent in situ hybridization (FISH). Co-expression with specific cell markers was assessed with double FISH; glutamatergic, gamma-aminobutyric acid (GABA)-ergic, glial, monoaminergic, as well as interneuron markers were tested. Automated quantification of the cells expressing the di

SUBMITTER: Immenschuh J 

PROVIDER: S-EPMC10474706 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature