Structural changes in perineuronal nets and their perforating GABAergic synapses precede motor coordination recovery post stroke.

ABSTRACT:

Background

Stroke remains one of the leading causes of long-term disability worldwide, and the development of effective restorative therapies is hindered by an incomplete understanding of intrinsic brain recovery mechanisms. Growing evidence indicates that the brain extracellular matrix (ECM) has major implications for neuroplasticity. Here we explored how perineuronal nets (PNNs), the facet-like ECM layers surrounding fast-spiking interneurons, contribute to neurological recovery after focal cerebral ischemia in mice with and without induced stroke tolerance.

Methods

We investigated the structural remodeling of PNNs after stroke using 3D superresolution stimulated emission depletion (STED) and structured illumination (SR-SIM) microscopy. Superresolution imaging allowed for

SUBMITTER: Dzyubenko E 

PROVIDER: S-EPMC10474719 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature