Rapid LC-MS/MS quantification of Organophosphate non-specific metabolites in hair using alkaline extraction approach.

ABSTRACT: Assessing human exposure to commonly used, highly toxic, but non-persistent organophosphates (OPs) is challenging because these toxicants are readily biotransformed into dialkyl phosphates (DAPs) and other metabolites. Growing hair accumulates toxicants and their metabolites, which makes hair a valuable non-invasively sampled matrix that can be used to retrospectively examine chemical exposure. However, the efficient quantification of hydrophilic DAP compounds in hair is challenging due to complex hair matrix effects. To improve upon existing methods, we first examined the acid dissociation constants (pKa) of DAPs and amino acids (major components in hair) and identified the best pH conditions for minimizing matrix effects. We hypothesized that under basic pH conditions DAPs and amino acid

SUBMITTER: Nguyen KN 

PROVIDER: S-EPMC10474783 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature