Multispectral in-line hologram reconstruction with aberration compensation applied to Gram-stained bacteria microscopy.

ABSTRACT: In multispectral digital in-line holographic microscopy (DIHM), aberrations of the optical system affect the repeatability of the reconstruction of transmittance, phase and morphology of the objects of interest. Here we address this issue first by model fitting calibration using transparent beads inserted in the sample. This step estimates the aberrations of the optical system as a function of the lateral position in the field of view and at each wavelength. Second, we use a regularized inverse problem approach (IPA) to reconstruct the transmittance and phase of objects of interest. Our method accounts for shift-variant chromatic and geometrical aberrations in the forward model. The multi-wavelength holograms are jointly reconstructed by favouring the colocalization of the object edges. Th

SUBMITTER: Brault D 

PROVIDER: S-EPMC10475072 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature