Efficient genome editing in erythroid cells unveils novel MYB target genes and regulatory functions.

ABSTRACT: Targeted genome editing holds great promise in biology. However, efficient genome modification, including gene knock-in (KI), remains an unattained goal in multiple cell types and loci due to poor transfection efficiencies and low target genes expression, impeding the positive selection of recombined cells. Here, we describe a genome editing approach to achieve efficient gene targeting using hard to transfect erythroid cell lines. We demonstrate robust fluorescent protein KI efficiency in low expressed transcription factor (TF) genes (e.g., Myb or Zeb1). We further show the ability to target two independent loci in individual cells, exemplified by MYB-GFP and NuMA-Cherry double KI, allowing multicolor labeling of regulatory factors at physiological endogenous levels. Our KI t

SUBMITTER: Deleuze V 

PROVIDER: S-EPMC10475484 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature