Parallel G-quadruplex recognition by neomycin.

ABSTRACT: G-quadruplex-forming nucleic acids have evolved to have applications in biology, drug design, sensing, and nanotechnology, to name a few. Together with the structural understanding, several attempts have been made to discover and design new classes of chemical agents that target these structures in the hope of using them as future therapeutics. Here, we report the binding of aminoglycosides, in particular neomycin, to parallel G-quadruplexes that exist as G-quadruplex monomers, dimers, or compounds that have the propensity to form dimeric G-quadruplex structures. Using a combination of calorimetric and spectroscopic studies, we show that neomycin binds to the parallel G-quadruplex with affinities in the range of K_a ∼ 10⁵-10⁸ M^-1, which depends on

SUBMITTER: Ranjan N 

PROVIDER: S-EPMC10475565 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature