Single-cell sequencing of individual retinal organoids reveals determinants of cell-fate heterogeneity.

ABSTRACT: With a critical need for more complete in vitro models of human development and disease, organoids hold immense potential. Their complex cellular composition makes single-cell sequencing of great utility; however, the limitation of current technologies to a handful of treatment conditions restricts their use in screens or studies of organoid heterogeneity. Here, we apply sci-Plex, a single-cell combinatorial indexing (sci)-based RNA sequencing (RNA-seq) multiplexing method to retinal organoids. We demonstrate that sci-Plex and 10× methods produce highly concordant cell-class compositions and then expand sci-Plex to analyze the cell-class composition of 410 organoids upon modulation of critical developmental pathways. Leveraging individual organoid data, we develop a method to measur

SUBMITTER: Tresenrider A 

PROVIDER: S-EPMC10475847 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature