Stromal-epithelial interaction induces GALNT14 in prostate carcinoma cells.

ABSTRACT:

Introduction

Cell-cell communication is an important process in healthy tissue but also gains enhanced attention regarding pathological tissue. To date, the tumor microenvironment is gradually brought into focus when studying tumorigenesis. In the prostate gland, stromal and epithelial cells greatly interact to maintain homeostasis or tissue integrity. This study focuses on an indirect communication via soluble factors.

Methods

To investigate the cell-cell interaction via soluble factors, the prostate carcinoma cell line LNCaP and the stromal primary cells p21 were co-cultured without direct contact and RNA was isolated at defined time points. Differences in gene expression were finally analyzed by RNA sequencing.

Results

RNA sequencing revealed a time-depending diffe

SUBMITTER: Czyrnik ED 

PROVIDER: S-EPMC10475991 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature