Workflow for Validating Specific Amino Acid Footprinting Reagents for Protein Higher Order Structure Elucidation.

ABSTRACT: Protein footprinting mass spectrometry probes protein higher order structure and dynamics by labeling amino acid side-chains or backbone amides as a function of solvent accessibility. One category of footprinting uses residue-specific, irreversible covalent modifications, affording flexibility of sample processing for bottom-up analysis. Although several specific amino acid footprinting technologies are becoming established in structural proteomics, there remains a need to assess fundamental properties of new reagents before their application. Often, footprinting reagents are applied to complex or novel protein systems soon after their discovery and sometimes without a thorough investigation of potential downsides of the reagent. In this work, we assemble and test a validation workflow tha

SUBMITTER: Moyle AB 

PROVIDER: S-EPMC10476636 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature