Protein higher-order-structure determination by fast photochemical oxidation of proteins and mass spectrometry analysis.

ABSTRACT: The higher-order structure (HOS) of proteins plays a critical role in their function; therefore, it is important to our understanding of their function that we have as much information as possible about their three-dimensional structure and how it changes with time. Mass spectrometry (MS) has become an important tool for determining protein HOS owing to its high throughput, mid-to-high spatial resolution, low sample amount requirement and broad compatibility with various protein systems. Modern MS-based protein HOS analysis relies, in part, on footprinting, where a reagent reacts 'to mark' the solvent-accessible surface of the protein, and MS-enabled proteomic analysis locates the modifications to afford a footprint. Fast photochemical oxidation of proteins (FPOP), first introduced in 2005

SUBMITTER: Liu XR 

PROVIDER: S-EPMC10476649 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature