Metabolic and functional remodeling of colonic macrophages in response to high-fat diet-induced obesity.

ABSTRACT: Little is known about the effects of high-fat diet (HFD)-induced obesity on resident colonic lamina propria (LP) macrophages (LPMs) function and metabolism. Here, we report that obesity and diabetes resulted in increased macrophage infiltration in the colon. These macrophages exhibited the residency phenotype CX3CR1^hiMHCII^hi and were CD4^-TIM4^-. During HFD, resident colonic LPM exhibited a lipid metabolism gene expression signature that overlapped that used to define lipid-associated macrophages (LAMs). Via single-cell RNA sequencing, we identified a sub-cluster of macrophages, increased in HFD, that were responsible for the LAM signature. Compared to other macrophages in the colon, these cells were characterized by elevated glycolysis, phagocytos

SUBMITTER: Castoldi A 

PROVIDER: S-EPMC10477064 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature