Natural killer cells modified with a Gpc3 aptamer enhance adoptive immunotherapy for hepatocellular carcinoma.

ABSTRACT:

Introduction

Natural killer cells can attack cancer cells without prior sensitization, but their clinical benefit is limited owing to their poor selectivity that is caused by the lack of specific receptors to target tumor cells. In this study, we aimed to endow NK cells with the ability to specifically target glypican-3⁺ tumor cells without producing cell damage or genetic alterations, and further evaluated their therapeutic efficiency.

Methods

NK cells were modified with a Gpc3 DNA aptamer on the cell surface via metabolic glycoengineering to endow NK cells with specific targeting ability. Then, the G-NK cells were evaluated for their specific targeting properties, cytotoxicity and secretion of cytokines in vitro. Finally, we investigated the therapeutic efficien

SUBMITTER: Zheng Y 

PROVIDER: S-EPMC10477160 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature