Improved pearl millet genomes representing the global heterotic pool offer a framework for molecular breeding applications.

ABSTRACT: High-quality reference genome assemblies, representative of global heterotic patterns, offer an ideal platform to accurately characterize and utilize genetic variation in the primary gene pool of hybrid crops. Here we report three platinum grade de-novo, near gap-free, chromosome-level reference genome assemblies from the active breeding germplasm in pearl millet with a high degree of contiguity, completeness, and accuracy. An improved Tift genome (Tift23D₂B₁-P1-P5) assembly has a contig N50 ~ 7,000-fold (126 Mb) compared to the previous version and better alignment in centromeric regions. Comparative genome analyses of these three lines clearly demonstrate a high level of collinearity and multiple structural variations, including inversions greater than 1 Mb. Differe

SUBMITTER: Ramu P 

PROVIDER: S-EPMC10477261 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature