Metagenomic sequencing of post-mortem tissue samples for the identification of pathogens associated with neonatal deaths.

ABSTRACT: Postmortem minimally invasive tissue sampling together with the detailed review of clinical records has been shown to be highly successful in determining the cause of neonatal deaths. However, conventional tests including traditional culture methods and nucleic acid amplification tests have periodically proven to be insufficient to detect the causative agent in the infectious deaths. In this study, metagenomic next generation sequencing was used to explore for putative pathogens associated with neonatal deaths in post-mortem blood and lung tissue samples, in Soweto, South Africa. Here we show that the metagenomic sequencing results corroborate the findings using conventional methods of culture and nucleic acid amplifications tests on post-mortem samples in detecting the pathogens attribute

SUBMITTER: Baillie VL 

PROVIDER: S-EPMC10477270 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature