Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system.

ABSTRACT: The development of the cardiac conduction system (CCS) is essential for correct heart function. However, critical details on the cell types populating the CCS in the mammalian heart during the development remain to be resolved. Using single-cell RNA sequencing, we generated a large dataset of transcriptomes of ~0.5 million individual cells isolated from murine hearts at six successive developmental corresponding to the early, middle and late stages of heart development. The dataset provides a powerful library for studying the development of the heart's CCS and other cardiac components. Our initial analysis identified distinct cell types between 20 to 26 cell types across different stages, of which ten are involved in forming the CCS. Our dataset allows researchers to reuse the datasets for

SUBMITTER: Ren H 

PROVIDER: S-EPMC10477280 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature