Celastrol Combats Methicillin-Resistant Staphylococcus aureus by Targeting Δ¹ -Pyrroline-5-Carboxylate Dehydrogenase.

ABSTRACT: The emergence and rapid spread of methicillin-resistant Staphylococcus aureus (MRSA) raise a critical need for alternative therapeutic options. New antibacterial drugs and targets are required to combat MRSA-associated infections. Based on this study, celastrol, a natural product from the roots of Tripterygium wilfordii Hook. f., effectively combats MRSA in vitro and in vivo. Multi-omics analysis suggests that the molecular mechanism of action of celastrol may be related to Δ¹ -pyrroline-5-carboxylate dehydrogenase (P5CDH). By comparing the properties of wild-type and rocA-deficient MRSA strains, it is demonstrated that P5CDH, the second enzyme of the proline catabolism pathway, is a tentative new target for antibacterial agents. Using molecular docking, bio-layer interferometry

SUBMITTER: Yuan Z 

PROVIDER: S-EPMC10477891 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature