Dissecting the effects of METTL3 on alternative splicing in prostate cancer.

ABSTRACT: Although the role of METTL3 has been extensively studied in many cancers, its role in isoform switching in prostate cancer (PCa) has been poorly explored. To investigate its role, we applied standard RNA-sequencing and long-read direct RNA-sequencing from Oxford Nanopore to examine how METTL3 affects alternative splicing (AS) in two PCa cell lines. By dissecting genome-wide METTL3-regulated AS events, we noted that two PCa cell lines (representing two different PCa subtypes, androgen-sensitive or resistant) behave differently in exon skipping and intron retention events following METTL3 depletion, suggesting AS heterogeneity in PCa. Moreover, we revealed that METTL3-regulated AS is dependent on N⁶-methyladenosine (m⁶A) and distinct splicing factors. Analysis of the AS

SUBMITTER: Wang L 

PROVIDER: S-EPMC10477979 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature