Extraction-free, one-pot CRISPR/Cas12a detection of microRNAs directly from extracellular vesicles.

ABSTRACT: Current methods for extracellular vesicle (EV) miRNA analysis mostly require RNA extraction, which results in a multi-step, time-consuming workflow. This study reports an extraction-free method that combines thermolysis treatment of EVs with a one-pot EXTRA-CRISPR assay, enabling the vastly simplified analysis of EV miRNAs with a comparable performance to that of the extraction-based assays.

SUBMITTER: Yan H 

PROVIDER: S-EPMC10478011 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature