Low-glucose culture environment can enhance the wound healing capability of diabetic adipose-derived stem cells.

ABSTRACT:

Background

Application of autologous adipose-derived stem cells (ASC) for diabetic chronic wounds has become an emerging treatment option. However, ASCs from diabetic individuals showed impaired cell function and suboptimal wound healing effects. We proposed that adopting a low-glucose level in the culture medium for diabetic ASCs may restore their pro-healing capabilities.

Methods

ASCs from diabetic humans and mice were retrieved and cultured in high-glucose (HG, 4.5 g/L) or low-glucose (LG, 1.0 g/L) conditions. Cell characteristics and functions were investigated in vitro. Moreover, we applied diabetic murine ASCs cultured in HG or LG condition to a wound healing model in diabetic mice to compare their healing capabilities in vivo.

Results

Human ASCs exhibited decre

SUBMITTER: Li CW 

PROVIDER: S-EPMC10478288 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature